Journal: mBio
Article Title: Carboxysome Mispositioning Alters Growth, Morphology, and Rubisco Level of the Cyanobacterium Synechococcus elongatus PCC 7942
doi: 10.1128/mBio.02696-20
Figure Lengend Snippet: Increased levels of Rubisco correlate with cell elongation and asymmetric cell division phenotypes. (A) Quantification of immunoblots against the Rubisco large subunit (RbcL) in each strain grown at 40°C in 2% CO 2 . (B) As in panel A, but cells were grown at 30°C. (C) As in panel A, but cells were grown at 20°C. RbcL signals were normalized to Atpβ content. (D) Quantification of RbcL in wild-type S. elongatus cells grown at the indicated temperatures in 2% CO 2 (gray) or no CO 2 added (red). In panels A to D, error bars represent the standard deviations from three independent biological replicates. Statistical significance was based on an unpaired t test. (E) Cell length quantification of wild-type cells when maintained at 2% CO 2 (+ CO 2 ) or down-shifted to ambient CO 2 (Down Shift). Wild-type S. elongatus cells elongate upon CO 2 down-shift. Statistical significance was based on a nonparametric Mann-Whitney test ( n ≥ 300 cells per condition). (F) Percentage of cells undergoing asymmetric division when maintained at 2% CO 2 (+ CO 2 ) or down-shifted to ambient CO 2 (Down Shift). Wild-type S. elongatus undergoes asymmetric cell division upon CO 2 down-shift. Error bars represent the standard deviations from at least three independent biological replicates ( n ≥ 300 dividing cells). Each dot represents the value from an independent field of view ( n ≥ 7 field of views per condition). Statistical significance was based on an unpaired t test and indicated as follows: ****, P < 0.0001; ***, P < 0.001; **, P < 0.01; *, P < 0.05; n.s., not significant.
Article Snippet: The membrane was immunoprobed using rabbit polyclonal antisera against RbcL and the beta subunit of ATP synthase, Atpβ (Agrisera) and then horseradish peroxidase (HRP)-conjugated anti-rabbit IgG secondary antibody (Millipore Sigma).
Techniques: Western Blot, MANN-WHITNEY
